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. 2018 Jun 11;11:198. doi: 10.3389/fnmol.2018.00198

Figure 1.

Figure 1

Cosmosiin increases the levels of ADAM10 protein without altering its mRNA level. (A) Dose-response effect of cosmosiin on the levels of ADAM10 protein in SH-SY5Y cells. Representative Western blots (left) showing a band near 80 kD and a band near 60 kD, representing the immature (im) and mature (m) forms of ADAM10, respectively; procedures were conducted using SH-SY5Y cells treated with cosmosiin (at 0.5, 1, 2.5, 5 and 10 μM) for 36 h (left). Bar plot summaries of im- and m-ADAM10 protein levels, and m-ADAM10/im-ADAM10 ratio, in the presence of cosmosiin (on the right). (B) Dose-response effect of cosmosiin on the levels of ADAM10 protein in HEK293 cells. Representative Western blots (left) and bar plot summaries (right) of the im-ADAM10 and m-ADAM10 protein levels, and m-ADAM10/im-ADAM10 ratio, in HEK293 cells treated with cosmosiin (0.5, 1, 2.5, 5 and 10 μM) for 36 h. (C) Time-course effect of cosmosiin on the levels of ADAM10 protein. Representative Western blots (left) and bar plot summaries (right) of the m-/im-ADAM10 in SH-SY5Y cells treated with 5 μM cosmosiin for indicated times (6, 12, 24, 36 and 48 h). (D) Relative ADAM10 mRNA levels in SH-SY5Y (left) and HEK293 (right) cells treated with cosmosiin (1, 5 and 10 μM) and apicidin (P, 0.25 μM) for 36 h. (E) Relative cell viability of SH-SY5Y (left) and HEK293 (right) cells treated with cosmosiin for 36 h. Data are expressed as means ± SD. *P < 0.05; **P < 0.01 (n = 4–5).