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. 2018 Jun 11;11:198. doi: 10.3389/fnmol.2018.00198

Figure 4.

Figure 4

Phosphatidylinositide 3-kinase (PI3K) signaling is involved in cosmosiin regulation of ADAM10. (A) Representative Western blots (top) and bar plot summary (bottom) of ADAM10 levels in SH-SY5Y cells treated with cosmosiin (5 μM) for 36 h and pre-incubated for 1 h with: ERK inhibitor U0126 (20 μM); PI3K inhibitors LY294002 (10 μM), wortmannin (0.1 μM), or 3-MA (50 μM). (B) Representative Western blots (top) and bar plot summary (bottom) of ADAM10 levels in SH-SY5Y cells treated with cosmosiin (5 μM) for 36 h and pre-incubated for 1 h with mTOR inhibitor rapamycin (RAPA, 5 μM). (C) Representative Western blots (top) and bar plot summary (bottom) of ERK and phosphorylated ERK (p-ERK) levels in SH-SY5Y cells in the absence or presence of cosmosiin (5 μM) or U0126 (20 μM) for 36 h. (D) Representative Western blots (top) and bar plot summary (bottom) of ERK and phosphorylated ERK (p-ERK) in SH-SY5Y cells treated with the vehicle DMSO (1:10,000) or 5 μM cosmosiin for indicated times (6, 12 and 24 h). (E) Representative Western blots (top) and bar plot summary (bottom) of AKT and phosphorylated AKT (p-AKT) in SH-SY5Y cells in the absence or presence of cosmosiin (5 μM) or LY294002 (10 μM) for 36 h. Data are expressed as means ± SD. *P < 0.05; **P < 0.01 (n = 3–4).