Fig. 2.

CHE targeting of the homologous gene locus. By analyzing the ECFP/non-DsRed progeny of tra^nCHE/+ males and tra^nDOCK/+ virgins, we focused on non-HDR targeting events at a single tra locus. Estimation of each genotype frequency based on the observed efficiency values is indicated above sperm illustrations. Molecular analysis of the CHE target site in F₁ female progeny (boxed) identified independent NHEJ-derived in-frame indels that resulted in drive-resistant functional alleles (SI Appendix, Fig. S5A).