Fig. 3.

Glyceroneogenesis and phosphatidylethanolamine (PE) levels are reduced by PEPCK-M silencing. (A) ¹³C₅-Glutamine was used as a tracer in H23 cells stably expressing nonsilencing shRNA (control shRNA, Ctrl-sh) or two different constructs of PEPCK-M shRNA (PEPCK-M-sh1 and PEPCK-M-sh2) in serum-free, low glucose (0.2 mM) medium. Enrichment of the label in the pool of GPL-bound glycerol is shown as mean ± SEM from four independent experiments. *P < 0.05, **P < 0.01. (B) Total levels of phospholipids in H23 cells stably expressing nonsilencing shRNA (Ctrl-sh) or two different constructs of PEPCK-M shRNA (PEPCK-M-sh1 and PEPCK-M-sh2) grown in 0.2 mM glucose for 96 h with daily medium replacement. Data are mean ± SEM from five independent experiments. (A and B) Group comparisons were performed with one-way ANOVA and Dunnett post hoc analysis versus Ctrl-sh.