Fig. 4.

PEPCK-M silencing substantially inhibits s.c. tumor growth. (A and B) Growth of s.c. tumors in nude mice (n = 8 animals per group) injected with H23 cells stably expressing control shRNA, or two different constructs of PEPCK-M shRNA (PEPCK-M-sh1 and PEPCK-M-sh2). (A) Tumor weight at the end of the experiment (median and interquartile range). P values were calculated by Mann–Whitney U test with Bonferroni correction for multiple comparisons. (B) Number of mice with macroscopic tumors, microscopic tumors found on histological examination, or without tumor. (C) Efficiency of PEPCK-M silencing in cells in vitro.