Fig. 4.

Noncoding RNA maturation by RNase Y and its dependency on the Y-complex. Rend-seq 5′-mapped (orange) and 3′-mapped (blue) read counts are plotted for the rnpB (A), scr (B), and rnaC (C) regions of the B. subtilis genome from wild-type (WT) cells (∆epsH background) and mutants lacking rny and ylbF. Mutants lacking YmcA or YaaT behave similarly (SI Appendix, Fig. S3). Dotted lines indicate the positions of mature 3′ ends. Zoomed-in views of 3′-mapped reads at the positions surrounding the mature 3′ ends are shown. (D) Rend-seq data from WT cells and mutants lacking rny and ylbF mapped to the B. subtilis 3610 plasmid pBS32 for the region antisense to comI. Dotted lines indicate the position of RNA ends that depend on the presence of RNase Y. RPM, reads per million.