Table 1.

Effects of Ala substitutions on stability

Interaction	Location	Tm, °C	ΔTm, °C*
Lateral DK	b:D16 c:K15A	30.8	3.3
	c:D10A a:K12	31.3	2.8
Lateral KD	c:K19 a:D21A	34.4	−0.3
Axial DK	a:D16 b:K18A	33.5	0.6
	c:D7A a:K12	35.0	−0.9
Axial KD	c:K19 a:D24A	31.2	2.9
	c:K13 a:D18A	28.6	5.5

Isolated charge-pair networks are highlighted in bold.

^*$\mathrm{\Delta }{T}_m=T_{m,\hspace{0.17em}abc}-T_{m,Ala.}$