Simplified phylogenetic tree of plant SEC1 proteins. The phylogenetic tree was generated using the neighbor-joining method in the CLC workbench program (abridged). Note that KEULE and SEC1B evolved differentially from SEC1A and that SEC1B is only present in dicot plants. Ambtr, Amborella trichopoda (basal dicot); Aquca, Aquilegia caerulea (basal angiosperm); Arath, Arabidopsis thaliana (Brassicaceae); Orysa, Oryza sativa (Monocot); Picab, Picea abies (gynmosperm); Spipo, Spirodela polyrhiza (basal monocot). See SI Appendix, Fig. S1 for a detailed phylogenetic tree.