Table 3.
MS analysis of synthetic SpaA pilus polymers
| HPLC retention time, min | Calculated MH+ | Predicted peptides | Mass accuracy, ppm | Pilus assembly reaction |
| 24.4 | 1947.0334 | DVHVYPKHQALS :: ELPLT | 4.0 | Reaction with SpaA30–500 |
| 26.5 | 1410.7627 | DVHVYPK* :: ELPLT | 2.8 | Reaction with SpaA30–500 |
| 22.9 | 1675.8802 | DVHVYPKHQ* :: ELPLT | 3.6 | Reaction with SpaA30–500 |
| 29.0 | 2336.2034 | DVHVYPKHQALS :: NAGFELPLT | 4.7 | Reaction with SpaA30–500 |
| 23.3 | 2400.2922 | DVHVYPKHQALSEPVK :: ELPLT | 2.9 | Reaction with SpaA30–500 |
| ND | 2485.2756 | DQITLITCTPYAVNSHR :: ELPLT | ND | Reaction with SpaA30–500 |
| ND | 2874.4455 | DQITLITCTPYAVNSHR :: NAGFELPLT | ND | Reaction with SpaA30–500 |
| ND | 1657.8717 | DQITLITCTP* :: ELPLT | ND | Reaction with SpaA30–500 |
| ND | 2047.0416 | DQITLITCTP* :: NAGFELPLT | ND | Reaction with SpaA30–500 |
| 24.5 | 1947.0334 | DVHVYPKHQALS :: ELPLT | 4.5 | Reaction with NSpaA and CSpaA |
| 26.5 | 1410.7627 | DVHVYPK :: ELPLT | 3.3 | Reaction with NSpaA and CSpaA |
| 22.9 | 1675.8802 | DVHVYPKHQ :: ELPLT | 3.5 | Reaction with NSpaA and CSpaA |
| ND | 2336.2034 | DVHVYPKHQALS :: NAGFELPLT | ND | Reaction with NSpaA and CSpaA |
| ND | 2400.2922 | DVHVYPKHQALSEPVK :: ELPLT | ND | Reaction with NSpaA and CSpaA |
| ND | 2485.2756 | DQITLITCTPYAVNSHR :: ELPLT | ND | Reaction with NSpaA and CSpaA |
| ND | 2874.4455 | DQITLITCTPYAVNSHR :: NAGFELPLT | ND | Reaction with NSpaA and CSpaA |
| ND | 1657.8717 | DQITLITCTP* :: ELPLT | ND | Reaction with NSpaA and CSpaA |
| ND | 2047.0416 | DQITLITCTP* :: NAGFELPLT | ND | Reaction with NSpaA and CSpaA |
| 36.5 | 1023.6448 | PKLI :: ELPLT | 11.9 | Reaction with CSpaA and SpaB |
MH+, the mass of the singly protonated species; ND, not determined.
*
Not expected cleavage sites.