Fig. 6.
Doc2-promoted augmentation is not mediated by changes in presynaptic Ca2+ dynamics. (A) Image of a representative neuron loaded with FM4-64 (magenta), to identify synaptic boutons, and Fluo-5F (heat map), to measure changes in [Ca2+]i. (B) Averaged Fluo-5F ∆F/F0 versus time traces in response to a single stimulation 5 s before (Upper) and 5 s after (Lower) the augmentation protocol were imaged using WT (340 boutons, four independent litters of mice) or Doc2α/β DKO (214 boutons, four independent litters of mice) neurons. (C) Scatterplots of Fpeak/F0 quantified from individual boutons. **P < 0.01, unpaired t test.