Table 3.
Targeted gene expression analysis using RT-qPCR.
| Gene (locus tag)a | Cysteine-deficient CDM | Methionine-deficient CDM |
|---|---|---|
| recA (02620) | 23.8 ± 0.6 | 23.6 ± 0.6 |
| rpoB (10445) | 18.3 ± 0.9 | 19.3 ± 0.9 |
| metQ1 (00255) | 25.5 ± 0.4 | 24.2 ± 0.5 |
| cysK (00530) | 22.3 ± 0.6 | 23.0 ± 0.8 |
| metC (00830) | 20.8 ± 0.4 | 18.0 ± 0.4 |
| yckJ (00840) | 24.6 ± 0.4 | 21.9 ± 0.5 |
| metE (00935) | 21.0 ± 0.4 | 17.7 ± 0.4 |
| yxjH (01620) | 22.1 ± 0.4 | 20.2 ± 0.8 |
| metK (02170) | 18.1 ± 1.1 | 18.2 ± 0.8 |
| metQ3 (04120) | 21.3 ± 0.3 | 19.8 ± 0.6 |
| glnP2 (14340) | 19.2 ± 0.3 | 22.9 ± 0.7 |
| glnM2 (14345) | 19.3 ± 0.8 | 22.4 ± 0.5 |
| artM2 (14350) | 18.7 ± 0.5 | 22.3 ± 0.5 |
| glnH4 (14355) | 17.6 ± 0.4 | 21.0 ± 0.8 |
| ctl1 (14660) | 21.4 ± 0.6 | 25.7 ± 0.6 |
The numbers represent the average of the quantification cycle value and standard deviation obtained from three independent experiments.
a
Numbers in brackets represent the locus_tag, of which the prefix FAM18149_ was omitted.