Figure 3.

Multivariate T cell analysis of mitogen-stimulated cytokine secretion profiles. (A) Unsupervised hierarchical clustering of mitogen-stimulated CD4⁺ and CD8⁺ T cell cytokine secretion profile show divergence based on non-tuberculous mycobacteria infection status. Each column represents an individual (patient/control) and each row represents a clustering variable (cytokine secretion pattern). Clustering patterns shown according to primary group [patient with cystic fibrosis (CF) black, or healthy control gray] and secondary group (CF^Act-light green, CF^Past-dark green, CF^Contro1-white, and HC^A-black). Clustering of CF^Ac and CF^Past patients with one exception and clustering of CF^Contro1 and HC^A with one exception. (B) Principle component analysis (PCA) and canonical co-variate analysis (CCA) show significant clustering (P = 0.02) of subjects by patient group. Marked overlap between CF^Act and CF^Past groups is seen in terms of global cytokine secretion profile indicating an overall similarity in secretion profile. CF^Contro1 group diverges further from CF^Act and CF^Past groups.