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. 2018 Jun;59(6):980–985. doi: 10.2967/jnumed.117.199075

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FIGURE 2. — Characterization of CD4 cells in colons and MLNs of DSS-treated mice. (A) Representative images of antimouse CD4 immunohistochemical staining in colons from control (top) and DSS-treated (bottom) mice. Left panels: ×4 magnification; scale bar, 500 μm. Right panels: ×10 magnification; scale bar, 200 μm. (B) Number of cells staining positively for CD4 from entire colon section divided by total number of cells. Two sections of colon, 100 μm apart, were analyzed per mouse, with each point representing average percentage of CD4+ T cells in colon from 1 mouse (control, n = 12 mice; DSS, n = 13). (C) Flow cytometric analysis of CD45+ CD4+ cells from MLNs (control, n = 9; DSS, n = 10) of control and DSS-treated mice. *P < 0.05.