Figure 2.

NaLTSA validation in permeabilized HeLa cells transiently expressing Nluc-MAPK14. (a) Samples were exposed to compounds [50 μM] and subjected to NaLTSA. (b) ΔT_m results obtained from n = 10 independent NaLTSAs to demonstrate reproducibility. (c) Melting curves for each [AMG 548]. (d) Isothermal dose response curves were generated by exposing duplicate sample plates to increasing [compound] at isothermal temperatures of 40 or 54 °C. (a,c,d) Shown are mean ± SEM, n = 4 independent experiments.