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. 2018 May 26;9(6):563–568. doi: 10.1021/acsmedchemlett.8b00191

Figure 3.

Figure 3

Development of ABC99yne, a clickable analogue of ABC99 for visualizing NOTUM activity in biological samples. (A) Structure of ABC99yne. (B) ABPP gel showing competitive blockade of FP-Rh labeling (left lanes) and direct labeling (right lanes) of NOTUM by ABC99yne in SW620 CM. Samples were pretreated with ABC99yne at the indicated concentrations (30 min, 37 °C) and then treated with FP-Rh (1 μM, 30 min, room temperature) or subjected to CuAAC conditions with a Rh–N3 tag prior to SDS-PAGE analysis.