FIGURE 5:

Cadherin-mediated cell–cell adhesion is required for cell surface retention of Tril in Xenopus explants. (A–D‴) RNA encoding Tril-HA (100 pg) was injected into a single animal pole blastomere of four-cell embryos. Ectoderm was explanted from 5–10 embryos in each group at stage 11 and immunostained for HA and Fibronectin (A–A‴) or E-cadherin (B–B‴). (C–C‴) RNA encoding Tri-HA (100 pg) and N-cad∆Emyc was coinjected into a single animal pole blastomere of four-cell embryos. Ectoderm was explanted from 5–10 embryos in each group at stage 11 and immunostained for HA and myc. Results were replicated in three independent experiments. A–C″″ show projections viewed from the xy-axis and A‴–D‴ viewed from the z-axis.