Figure 6.

Substrate-immobilized CCL21 + ICAM1 increase T-cell expression of Granzyme B and PD-1, while not affecting FasL. (A–C) Bar graph illustrating the mean fluorescence intensity of granzyme B (A), FasL (B), and PD-1 (C) in cytotoxic T-cells following their incubation for 24 and 48 h with B16-ovalbumin target cells. T-cells were pre-cultured for 7 days on either an uncoated substrate, or on substrate-immobilized CCL21 + ICAM1 [data are from one experiment representative of three independent experiments with four replicates each (see Figure S4 in Supplementary Material). Error bars represent SEM. Calculated p-values (using standard t-test) are as indicated in the Figure].