Figure 7.

Substrate-immobilized CCL21 + intercellular adhesion molecule 1 (ICAM1) augment tumor suppression by CD8⁺ T-cells in vivo. (A,B) C57BL/6 mice were injected with 2 × 10⁶ B16 cells expressing ovalbumin and GFP. Seven days later, tumor-bearing mice were split into treatment groups, according to similar tumor average size and distribution, and injected intravenously with 2 × 10⁶ (A) or 4 × 10⁶ activated OT-I T-cells (B). Averages of measured tumor volumes are shown for either untreated mice (gray), or for mice treated with OT-I T-cells pre-cultured for 7 days on an uncoated substrate (blue), or on substrate-immobilized CCL21 + ICAM1 (pink). (C1–D2) Images of histology sections of three representative tumors of mice treated with 2 × 10⁶ OT-I T-cells pre-cultured on uncoated substrates (D1), or on substrate-immobilized CCL21 + ICAM1 (D2); or treated with 4 × 10⁶ OT-I T-cells pre-cultured on uncoated substrates (E1), or on substrate-immobilized CCL21 + ICAM1 coating (E2). Scale bar: 5 mm. T-cells pre-cultured on substrate-immobilized CCL21 + ICAM1 suppressed tumor growth to a significantly greater extent than T-cells cultured on uncoated substrates. N = 10 mice per group. Error bars represent SEM.