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. 2018 Apr 26;15(6):9889–9895. doi: 10.3892/ol.2018.8579

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Copyright: © Lu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — p62 expression in osteosarcoma cells and shRNA-mediated suppression. (A) p62 mRNA expression in F5M2 and F4 human osteosarcoma cells and control hFOB1.19 normal human osteoblast cells detected using the reverse transcription-quantitative polymerase chain reaction. All osteosarcoma cells expressed increased p62 mRNA levels compared with the normal osteoblasts. (B) Western blotting results demonstrating p62 protein expression in F5M2 and F4 osteosarcoma cells and control hFOB1.19 normal human osteoblast cells. (C) Relative p62 expression levels as analyzed using ImageJ software. All osteosarcoma cells expressed an increased amount of p62 protein compared with normal osteoblasts. (D) p62 protein detection by western blotting following shRNA silencing. (E) p62 relative protein expression levels analyzed using ImageJ. p62 expression was efficiently suppressed by shRNA in F5M2 and F4 cells. ***P<0.001. Data are presented as the mean ± standard deviation of three independent experiments. sh, short hairpin.