Figure 3.
Effects of Ace and/or Cis treatment on PCNA expression in Hep-2 cells. (A) PCNA expression in Hep-2 cells was determined by western blot analysis. Protein loading was assessed by probing for β-actin. Experiments were duplicated in independent cultures. Representative blot images are shown. (B) Quantification of PCNA protein expression levels following drug treatments for 48 h. Data expressed as mean ± standard deviation. *P<0.05 and **P<0.01 compared with control group; #P<0.05 compared with Cis group; &P<0.05 compared with AceH group. Ace, acetazolamide; Cis, cisplatin; PCNA, proliferating cell nuclear antigen; L, low dose 1×10−8 mol/l; H, high dose 5×10−8 mol/l.