Figure 5.
Effects of Ace and/or Cis treatment on the expression of apoptosis-related proteins. (A) Bcl-2, bax and caspase-3 expression in Hep-2 cells was determined by western blot analysis. Protein loading was assessed by probing for β-actin. (B) Quantification of the bcl-2/bax protein expression ratio following drug treatments for 48 h. (C) Quantification of caspase-3 protein expression levels following drug treatments for 48 h. Data expressed as mean ± standard deviation. *P<0.05 and **P<0.01 compared with control group; #P<0.05 and ##P<0.01 compared with Cis group; &P<0.05 compared with AceH group. Ace, acetazolamide; Cis, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X; L, low dose 1×10−8 mol/l; H, high dose 5×10−8 mol/l.