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. 2018 Apr 17;15(6):9025–9032. doi: 10.3892/ol.2018.8511

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Copyright: © Shao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Knockdown of LSD1 inhibits cell-cycle progression via the G1 phase. (A) LSD1-KD cells were treated with 100 ng/ml Dox for 48 h. Flow cytometric analysis (left and middle panels) and quantitation (right panel) indicated that knockdown of LSD1 significantly induced cell cycle arrest in the G1 phase. (B) Reverse transcription-quantitative polymerase chain reaction analysis revealed a decrease in cyclin D1 and CDK2 and an increase in p21^Cip1 mRNA expression levels. Data represented as the mean ± standard error of the mean (n=3). The data were analyzed by Student's t-test (*P<0.05). CDK2, cyclin-dependent kinase 2; Dox, doxycycline; LSD1, lysine-specific demethylase 1; LSD1-KD, Dox-mediated LSD1 knockdown of cells transduced with pLKO-LSD1-short hairpin RNA; p21^Cip1, cyclin dependent kinase inhibitor 1.