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. 2018 Apr 4;15(6):8505–8515. doi: 10.3892/ol.2018.8430

Figure 2.

Figure 2.

Hepa1-6 cells treated with HBx plus irradiation induced maturation of DCs. DCs were generated from C57BL/6 mouse bone marrow and were cultured in RPMI-1640 with recombinant murine GM-CSF. After plused with NS, irradiated Adnull-infected Hepa1-6 cells, irradiated Hepa1-6 cells or irradiated AdHBx-infected Hepa1-6 cells for 6 h, the cells were collected and washed with PBS, then stained with anti-CD40-FITC, anti-PD-L1-FITC, anti-CD80-FITC, and anti-CD86-FITC for phenotype analysis. Inhibition of autophagy by 3-MA significantly abolished the maturation of DCs (blank: isotype, pink: sample). (A) Representative results of 3 independent experiments are shown. Percentage of the DCs with the expression of each key surface molecules were shown in bottom of each corresponding rank as mean ± SEM (*P<0.05). The production of IL-12 and IFN-γ by the DCs after treatment with NS, irradiated Adnull-infected Hepa1-6 cells, irradiated Hepa1-6 cells, irradiated AdHBx-infected Hepa1-6 cells or irradiated AdHBx-infected Hepa1-6 cells + 3-MA. (B) ELISA assay shown that IL-12 and IFN-γ was released in significantly higher mounts in vaccine pulsed DC group than control groups (*P<0.05).