Figure 4.
Calreticulin, HMGB1 and HSP70 are selectively released by dying tumor cells. Calreticulin, HMGB1 and HSP70 were detected by western blot analysis in whole-cell lysates of irradiated Hepa1-6 cells, irradiated Adnull-infected Hepa1-6 cells, irradiated Hepa1-6 cells, irradiated AdHBx-infected Hepa1-6 cells or irradiated AdHBx-infected Hepa1-6 cells co-treated with 3-MA. Calreticulin, HMGB1 and HSP70 were increased in irradiated AdVEGFR2-infected 4T1 cells.