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(A) Proteins bands, as obtained by western blotting. (B) Histogram illustrates the protein expression levels of SphK1, ERK1/2 and p-ERK1/2 in SphK1(+)-HT-29 and NC-HT-29 cells. GAPDH was used as the reference gene. Data are presented as the mean ± standard deviation. (*P<0.05 vs. NC-HT-29). SphK1, sphingosine kinase 1; NC, negative control; p-, phosphorylated; ERK, extracellular signal-regulated kinase.
