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(A) Protein bands, as obtained by western blotting. (B) Quantification of the western blot bands illustrate the protein expression levels of LC3A, ATG5 and ULK1 in SphK1(+)-HT-29 and NC-HT-29 cells. GAPDH was used as the reference gene. Data are presented as the mean ± standard deviation. (*P<0.05 vs. NC-HT-29). SphK1, sphingosine kinase 1; NC, negative control.
