Figure 2.

Location and expression levels of CBS, CSE and MPST in human urothelial cell lines. (A) CBS, CSE and MPST immunofluorescence in the SV-HUC-1 normal bladder urothelium and the EJ bladder cancer cell lines. Magnification, ×100. (B) Protein expression levels of CBS, CSE and MPST in urothelial cell lines as determined by western blotting. (C) H²S production in urothelial cell lines. H²S productivity was the highest in EJ cells (P=0.003). **P<0.01 vs. SV-HUC-1 cells. CBS, cystathionine β-synthase; CSE, cystathionine γ-lyase; MPST, 3-mercaptopyruvate sulfurtransferase.