Table 1.
Carbohydrate-active enzymes identified in the metasecretome of a wheat straw degrading compost-derived community
| Protein ID | Predicted domaina | Predicted CAZy class | Description | Hit accession | E-value | Seq ID (%) | Coverage (%) | Taxonomic affiliation |
Taxonomic kingdom |
|---|---|---|---|---|---|---|---|---|---|
| Proteins unique to biotin-labelled fraction | |||||||||
| c134278_g1_i1_1 | CBM2-CBM60-GH10 | GH | Beta-1,4-xylanase | BAD88441 | 0 | 86 | 73 | Pseudomonas | Bacteria |
| c155626_g1_i1_1 | CE10 | CE | Esterase | WP_051322721 | 4E-152 | 77 | 100 | Luteimonas | Bacteria |
| c155626_g1_i1_4 | GH51 | GH | Alpha-N-arabinofuranosidase | WP_024870432 | 0 | 80 | 100 | Pseudoxanthomonas | Bacteria |
| c180274_g1_i1_4 | GH5 | GH | Endoglucanase | AIF91557 | 0 | 61 | 83 | Alteromonadaceae | Bacteria |
| c180864_g1_i1_2 | CBM2-CBM22-GH10 | GH | Endo-1,4-beta-xylanase | WP_007643754 | 0 | 71 | 99 | Cellvibrio | Bacteria |
| c189421_g1_i1_1 | GH6 | GH | Cellobiohydrolase | WP_013117127 | 0 | 95 | 92 | Cellulomonas | Bacteria |
| c190646_g3_i1_5 | CBM2-CBM10-GH6 | GH | Cellobiohydrolase | ACE85978 | 0 | 67 | 100 | Cellvibrio | Bacteria |
| c190817_g1_i1_7 | CBM44 | CBM | Hypothetical protein | WP_052773020 | 0 | 65 | 90 | Luteimonas | Bacteria |
| c192149_g1_i1_4 | CE10 | CE | Peptidase S9 | WP_027072364 | 0 | 79 | 91 | Luteimonas | Bacteria |
| c204578_g3_i1_1 | CBM2-CBM22 | CBM | Endo-1,4-beta-xylanase | WP_007643754 | 0 | 84 | 98 | Cellvibrio | Bacteria |
| c207114_g1_i1_5 | GH38 | GH | Hypothetical protein | WP_051602586 | 5E-111 | 35 | 85 | Hyphomonas | Bacteria |
| c207852_g16_i3_8 | CE10 | CE | Hypothetical protein | WP_027072315 | 0 | 84 | 95 | Luteimonas | Bacteria |
| c208411_g1_i3_1 | CBM61 | CBM | Subtilisin-like proprotein | WP_014780843 | 1E-18 | 37 | 25 | Aequorivita | Bacteria |
| c208914_g1_i1_3 | CE10 | CE | Unknown | CDW81391 | 3E-42 | 64 | 98 | Stylonychia | Eukaryota |
| c209736_g2_i3_67 | GT4 | GT | Hypothetical protein | WP_005674657 | 1E-115 | 49 | 99 | Lautropia | Bacteria |
| c209807_g2_i1_6 | SLH | SLH | S-layer protein | WP_013176560 | 2E-166 | 37 | 100 | Truepera | Bacteria |
| c211555_g1_i1_2 | GH11-CBM60 | GH | 1,4-beta-xylanase | WP_049629015 | 0 | 81 | 100 | Cellvibrio | Bacteria |
| c27111_g1_i1_1 | CBM2-CBM2 | CBM | Hypothetical protein | ESQ13017 | 7E-111 | 39 | 99 | Uncultured desulfofustis | Bacteria |
| c344648_g1_i1_2 | CBM50 | CBM | Peptidoglycan-binding protein | WP_027071529 | 0 | 88 | 99 | Luteimonas | Bacteria |
| c345827_g1_i1_2 | CE15 | CE | Hypothetical protein | WP_012238067 | 0 | 68 | 83 | Sorangium | Bacteria |
| c528853_g1_i1_2 | CBM44 | CBM | CARDB domain-containing protein | AEV33755 | 4E-52 | 34 | 51 | Owenweeksia | Bacteria |
| c711977_g1_i1_3 | SLH | SLH | Hypothetical protein | WP_009455020 | 1E-14 | 31 | 29 | Fischerella | Bacteria |
| c724886_g1_i1_1 | CBM2-GH5 | GH | Endo-1,4-beta-D-glucanase | ACY24859 | 0 | 81 | 100 | Uncultured microorganism | |
| c80983_g1_i1_2 | CBM2-CBM60-CE1 | CE | Hypothetical protein | WP_051234546 | 0 | 74 | 99 | Marinimicrobium | Bacteria |
| c34457_g1_i1_2 | AA2 | AA | Catalase/peroxidase HPI | WP_036397899.1 | 2E-78 | 1 | 0.9 | Mycobacterium | Bacteria |
| c210210_g2_i1_1 | GH109 | GH | gfo/Idh/MocA family oxidoreductase | WP_006979342.1 | 9E-151 | 0.99 | 0.52 | Chthoniobacter | Bacteria |
| c350217_g1_i1_3 | AA6 | AA | NADPH-dependent FMN reductase | WP_027072629.1 | 3E-100 | 1 | 0.8 | Chthoniobacter | Bacteria |
| c180629_g1_i1_1 | GH25 | GH | glycosyl hydrolase family 25 protein | XP_001008527.1 | 6E-64 | 0.98 | 0.5 | Tetrahymena | Eukaryota |
| c190646_g2_i1_3 | CBM2 | CBM | Cellobiohydrolase | WP_007642349.1 | 0 | 1 | 0.74 | Cellvibrio | Bacteria |
| c208441_g1_i1_2 | GH3 | GH | 1,4-beta-D-glucan glucohydrolase | WP_027070958.1 | 0 | 0.97 | 0.82 | Luteimonas | Bacteria |
| c151435_g1_i1_2 | GH5 | GH | Endoglucanase | WP_084618390.1 | 0 | 1 | 0.74 | Cellvibrio | Bacteria |
| c199479_g2_i1_4 | CBM2 | CBM | DUF1592 domain-containing protein | WP_007644728.1 | 0 | 1 | 0.78 | Cellvibrio | Bacteria |
| c207123_g9_i1_7 | CE1 | CE | S9 family peptidase | WP_043740359.1 | 0 | 0.95 | 0.87 | Luteimonas | Bacteria |
| c349698_g1_i1_1 | GH9 | GH | Glycoside hydrolase | WP_041523229.1 | 0 | 1 | 0.7 | Gilvimarinus | Bacteria |
| c203693_g1_i1_7 | CBM4 | CBM | Cellulose 1,4-beta-cellobiosidase | WP_085113009.1 | 7E-19 | 0.39 | 0.26 | Thermoanaerobacterium | Bacteria |
| c225675_g1_i1_1 | CBM44 | CBM | T9SS C-terminal target domain-containing protein | WP_041627631 | 2E-52 | 32 | 59 | Owenweeksia | Bacteria |
| Proteins unique to supernatant fraction | |||||||||
| c159637_g1_i1_5 | DOCKERIN | Dockerin | n.d | ||||||
| c205510_g2_i1_1 | GH74 | GH | T9SS C-terminal target domain-containing protein | WP_027376910 | 7E-41 | 55 | 100 | Chryseobacterium | Bacteria |
| c38599_g1_i1_1 | CBM37 | CBM | Hypothetical protein | WP_035755077.1 | 2E-142 | 1 | 0.55 | Flavobacterium | Bacteria |
| c203621_g1_i1_2 | CBM6 | CBM | T9SS C-terminal target domain-containing protein | WP_040481137.1 | 1E-86 | 0.32 | 0.5 | Mariniradius | Bacteria |
| c185673_g1_i1_1 | AA6 | AA | NADPH-dependent oxidoreductase | WP_046482965.1 | 9E-109 | 0.99 | 0.81 | Pseudomonas | Bacteria |
| c208949_g1_i1_1 | PL9-PL9 | PL | Nitrous oxidase accessory protein | WP_014202187 | 3E-19 | 25 | 30 | Owenweeksia | Bacteria |
| Proteins common between biotin-labelled and supernatant fractions | |||||||||
| c155243_g1_i1_4 | GH38 | GH | TonB-dependent receptor | WP_052633156 | 0 | 79 | 99 | Pseudoxanthomonas | Bacteria |
| c186013_g1_i1_1 | GH6 | GH | Cellobiohydrolase | WP_027328555 | 0 | 77 | 84 | Marinimicrobium | Bacteria |
| c194919_g2_i1_5 | CBM2 | CBM | Carbohydrate-binding protein | WP_007644728 | 0 | 77 | 99 | Cellvibrio | Bacteria |
| c208473_g1_i1_1 | CE8-CBM37 | CBM | T9SS C-terminal target domain-containing protein | WP_028522307 | 4E-74 | 55 | 49 | Runella | Bacteria |
| c194919_g2_i1_4 | CBM2 | CBM | YceI family protein | WP_087469052.1 | 0 | 0.51 | 1 | Cellvibrio | Bacteria |
| c209441_g2_i1_1 | AA7 | AA | FAD-binding protein | WP_053231510.1 | 6E-164 | 0.93 | 0.51 | Sandaracinus | Bacteria |
| c711379_g1_i1_1 | GH74 | GH | T9SS C-terminal target domain-containing protein | WP_084016764.1 | 0 | 1 | 0.94 | Moheibacter | Bacteria |
| c531189_g1_i1_2 | CE10 | CE | Hypothetical protein | PCJ83084.1 | 5E-116 | 1 | 0.39 | Flavobacteriales | Bacteria |
| c205510_g1_i1_1 | GH74 | GH | T9SS C-terminal target domain-containing protein | WP_084016764.1 | 0 | 1 | 0.83 | Moheibacter | Bacteria |
| c63252_g1_i1_3 | GH48 | GH | Exoglucanase | WP_013118318.1 | 0 | 0.93 | 0.93 | Cellulomonas | Bacteria |
| c349698_g1_i1_5 | CE1 | CE | Feruloyl esterase | WP_072812830.1 | 2E-67 | 0.88 | 0.43 | Fibrobacter | Bacteria |
| c540340_g1_i1_1 | CBM44 | CBM | T9SS C-terminal target domain-containing protein | WP_070137948.1 | 1E-42 | 0.56 | 0.6 | Crocinitomix | Bacteria |
| c190535_g1_i1_1 | SLH | SLH | Hypothetical protein | OGI23348.1 | 2E-140 | 1 | 0.39 | n.d | Bacteria |
| c600504_g1_i1_1 | CE8 | CE | n.d | ||||||
aDomain structure for each protein was predicted based on CAZy database searches