Miltirone induces Bax, AIF and p53 protein expression in HCC827 and A549 cisplatin-resistant lung cancer cells. (A) The effect of miltirone on Bax, AIF and p53 protein expression was determined using western blotting in HCC827 and A549 cisplatin-resistant lung cancer cells. The statistical analysis of (B) Bax, (C) AIF and (D) p53 protein expression. GAPDH served as a loading control. **P<0.01 vs. the untreated control (0 µM miltirone). Bax, B-cell lymphoma 2-associated X-protein; AIF, apoptosis-inducing factor.