Figure 3.

Identified differentially expressed proteins verification by RT-qPCR and western blot analysis. (A) MicroRNA levels of 8 upregulated proteins (MYH9, CD44, ITGB1, LAMB3, PHGDH, ACTN4, LMNA and VIM) and 8 downregulated proteins (PSMA7, MTHFD1, GSTM3, FH, HSPB1, LRPPRC, CPT2 and IDH2) were determined by RT-qPCR following the transfection of SPC-A-1 cells with an miR-148a inhibitor or control oligonucleotide. β-actin served as an internal control. (B) Protein levels of LAMB3, VIM, PHGDH, ACTN4, FH, HSPB1, IDH2, LDHB, FASN and CAT were determined by western blot analysis following the transfection of SPC-A-1 cells with a miR-148a inhibitor or control oligonucleotide. β-actin served as an internal control. The data were representative of three independent experiments. *P<0.05. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; MYH9, myosin heavy chain 9; CD44, cluster of differentiation 44; ITGB1, integrin β1; LAMB3, laminin subunit β3; PHGDH, phosphoglycerate dehydrogenase; ACTN4, α-actinin-4; LMNA, lamin A/C; VIM, vimentin; PSMA7, proteasome subunit α7; MTHFD1, methylenetetrahydrofolate dehydrogenase; GSTM3, glutathione S-transferase M3; FH, fumarate hydratase; HSPB1, heat shock protein β1; LRPPRC, leucine rich pentatricopeptide repeat; CPT2, carnitine palmitoyltransferase 2; IDH2, isocitrate dehydrogenase 2; LDHB, lactate dehydrogenase B; FASN, fatty acid synthase; CAT, catalase.