Figure 3.

Effect of 6-O-angeloylplenolin on protein expression of MDR1 and activity of the MDR1 promoter. (A) HCT-8/VCR cells were treated with 6-O-angeloylplenolin for 24 h, and flow cytometry assay was performed (n=4). *P<0.05 and **P<0.01 vs. untreated HCT-8/VCR cells. (B) Cells were treated with 6-O-angeloylplenolin for 2 days following co-transfection with pGL3-MDR and pRL-TK. The activity of MDR1 promoter was measured using a dual-luciferase assay. Data are represented as mean ± standard deviation (n=4). *P<0.05 and **P<0.01 vs. untreated HCT-8/VCR cells; ^||P<0.01 was compared with untreated HCT-8 cells. MDR1, ATP-binding cassette sub-family B member 1.