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. 2018 Apr 10;15(6):8735–8743. doi: 10.3892/ol.2018.8447

Figure 1.

Figure 1.

BSO suppresses glutathione biosynthesis and decreases cell viability. (A) Enzymatic inhibition of GCL by BSO. (B-F) Cellular effects of BSO in PANC-1 cells. (B) GSH+GSSG levels, normalized by cellular ATP, determined following incubation with BSO for 24 h. (C) Induction of lipid peroxidation by 100 µM BSO, and attenuation by 10 mM NAC and 100 µM α-tocopherol. (D) BSO-induced decrease in cell viability, and rescue effects of (E) GSHee and (F) NAC. (A, n=4 and D, n=3) Data are presented as the mean ± SD. (B, E and F) Data are presented as the mean ± SD (n=3); ***P<0.0005 using Williams' test. BSO, buthionine sulfoximine; GCL, glutamate-cysteine ligase; GSH, glutathione (reduced form); GSSG, glutathione disulphide; ATP, adenosine triphosphate; NAC, N-acetylcysteine; GSHee, GSH monoethyl ester; SD, standard deviation; BODIPY, boron dipyrromethene.