Figure 3.
Expression of 53BP1 is associated with cisplatin chemoresistance. (A) Cell viability was assayed following treatment with an increasing concentration of cisplatin for 72 h. (B) Expression of p-Akt, Bax, Bcl-2, CDK2 and p21 in SKOV3/pLPC-53BP1 and SKOV3/pLPC-vector cells following treatment with 0, 0.4, 0.8, 1.6 µg/ml cisplatin was assessed using western blot analysis. β-actin served as a loading control. The relative expression of (C) p-Akt, (D) Bax/Bcl-2, (E) CDK2 and (F) p21 in SKOV3/pLPC-53BP1 and SKOV3/pLPC-vector cells. β-actin was a normalization control. Data were represented as the mean ± standard deviation. 53BP1, tumor protein p53 binding protein 1; p-Akt, phosphorylated protein kinase B; Bax, Bcl-2 associated X; Bcl-2, B cell lymphoma-2; CDK2, cyclin dependent kinase 2.