Figure 3.

HMGA1 is the target of miR-26a and inhibited by miR-26a. (A) The binding site of HMGA1 for the miR26a was predicted by the TargetScan website. The mutated nucleotides are indicated in red. (B) The U2OS cells were co-transfected with the pmir-GLO-HMGA1-3′UTR (WT) or pmir-GLO-HMGA1-3′UTR (MT) luciferase reporter and miR-26a mimic. The luciferase activity was measured using the One-Glo luciferase assay instrument (*P<0.05 vs. control mimic). (C) The U2OS cells was transfected with miR-26a mimic and inhibitor. The protein levels of HMGA1 in U2OS cells were determined using western blot analysis. HMGA1, targeting high-mobility group A1; U2OS, two osteosarcoma cell line; WT, wild-type; MUT, mutant-type.