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miR-28-5p inhibits the phosphorylation of AKT in gastric cancer cells. The phosphorylation levels of ERK, Pak1, Pak4 and AKT in BGC823 and SGC7901 cells overexpressing miR-28-5p and control cells were detected by western blot analysis. GAPDH served as protein loading control. AKT, RAC serine/threonine-protein kinase; P-ERK, phosphorylated extracellular signal-regulated kinase; Pak1, p21-activated kinase 1; miR-28-5p, microRNA-28-5p.
