Table 2. Melting temperatures of DNA 3WJs and the DNA mixture in the absence and presence of Ni^II ions ^a .

	Metal-free b	1 eq. of NiII
	T m/°C	T m/°C	ΔTm/°C
Three-way junctions
L1L2L3	51.7 ± 0.3	70.5 ± 0.4	+18.8
S1S2S3	42.1 ± 0.3	42.6 ± 0.5	+0.5
S4S5S6	43.3 ± 0.5	43.0 ± 0.4	–0.3
Mixture of six strands
L1, L2, L3, S4, S5, and S6	62.5 ± 0.3	43.7 ± 0.7	–18.8
		69.3 ± 0.6	+6.8

^aAverage of at least 3 runs after annealing. Standard errors are also listed.

^bIn the presence of 10 equiv. of EDTA.