A) On day 21 after saline or bleomycin aspiration, mice were euthanized, and after collecting BAL fluid, lungs were sectioned and stained for collagen with PicroSirius red. Images are representative of three or four mice per group. Bar is 200 μm. B) Quantification of PicroSirius red staining. Values are mean ± SEM, n = 3–4 mice per group. * p < 0.05, ** p < 0.01 (1-way ANOVA, Tukey’s test). C) Lung sections were stained for collagen-VI (green). Nuclei were counterstained with DAPI (blue). D) Higher magnification images of lung sections stained for collagen_VI (green) or irrelevant rabbit antibodies and DAPI (blue). Bar is 200 μm. Images are representative of three independent experiments.