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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Clin Cancer Res. 2018 Jan 8;24(6):1402–1414. doi: 10.1158/1078-0432.CCR-17-2074

Figure 5. RB Mediates FOXM1 and Survivin Transcription in NSCLC.

Figure 5

(A) Schematic illustration shows the location of three putative RB/E2F binding sites on FOXM1 and Survivin upstream sequence (promoters). (B & C left panels) Real-time PCR analysis of α-RB Chromatin immunoprecipitation assay shows the recruitment of RB on RB/E2F binding site II on FOXM1 and Survivin promoter, mouse α-GFP served as a negative control and input served as a positive control in shCon and shRB H1299 cells in response CDK4/6 inhibition (500 nM). (B & C right panels) PCR analysis of α-ACH4 chromatin immunoprecipitation assay shows that RB fails to recruit on RB/E2F binding site II on acetylated FOXM1 and Survivin promoter. Rabbit IgG served as a negative control and input served as a positive control in shCon and shRB cells in response to CDK4/6 inhibition (500 nM). (D) Immunoblotting analysis of FOXM1 Survivin, E2F1, E2F2, E2F3 and LaminB in ectopically expressed adenovirus harboring E2F1, E2F2, E2F3 cDNA in H1299 and H460 cells. Each data point is a mean ± SD from three or more independent experiments. **p <0.05 were considered as statistically significant.