(A) Schematic of experiment: same as in Figure
6A, but with analysis (B)–(D) limited to whisker-responsive
units.
(B) Spike rasters (first 100 trials) and PSTHs (95% CIs) showing
responses to horizontal (left column) or vertical (right) whisker stimulations
for a wS2 unit with (green) and without (black) inhibition of
S2L4.
(C) Top: spike rate during horizontal versus vertical whisker deflections (means
± 95% CIs) for the example unit in (B). Bottom: change in
orientation sensitivity index (OSI; ±95% CI)
due to optogenetic inhibition for same unit.
(D) Change in OSI plotted for each whisker-responsive wS2 unit
as a function of normalized depth within cortex. Conventions as in Figure 5F. Example unit from (B) indicated by
dark blue filled circle. Pie charts show percentages of wS2 units with
significant change in OSI during S2L4 optogenetic
inhibition (n = 467 whisker-responsive units from 6 mice).
(E) Schematic of experiment: same as in Figure
6E, but with analysis (F)–(H) limited to whisker-responsive
units.
(F and G) Same as (B) and (C) but for a wS1 unit.
(H) Same as (D) but for wS1 units (n = 398 whisker-responsive units from
7 mice). Example unit from (F) indicated by dark blue filled circle.
(I) Left: change in mean spike rate during inhibition of S2L4 for
preferred and non-preferred whisker deflection orientations, for those wS2 units
(filled circles from D) showing significantly increased (red; n = 12) or
decreased (blue; n = 18) OSI. Right: same data as in
left panel but expressed as percent change in firing rate.
(J) Same as (I) but for wS1 units (n = 3 and 34 units for increased and
decreased OSI, respectively).
See also Figure S7.