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. 2018 Jun 8;64(1):205–221. doi: 10.3233/JAD-180054

Fig.6.

Fig.6

Pyk2 is activated by Fyn in vivo. A) Immunoblots of lysates obtained from Pyk2-V5- and Fyn-Myc-transfected HEK293T cells. Mock, transfection with Lipofectamine only. Sh, short exposure; lo, long exposure. B) Quantification of immunoblots in (A) suggests that increased activity of Pyk2 is mediated by Fyn. Mean±s.e.m, n = 3 per group, two-tailed t test, n.s., not significant; **p < 0.01; ***p < 0.001. C) Immunoblots of HEK293T cell lysates demonstrating that Pyk2 activation is Fyn activity-dependent. FynCA-Myc, constitutively active mutant Fyn (Y531F); FynKD-Myc, kinase-dead mutant Fyn (K299A). # denotes endogenous Fyn. D) Immunoblots of whole brain lysates obtained from 3-week-old Fyn CA transgenic mice and littermate controls. FynCA Tg, transgenic mice neuronally overexpress a constitutively active mutant Fyn (Y531F). E) Quantification of immunoblots in (D) suggests that the increased activity of Pyk2 is mediated by Fyn. Mean±s.e.m, n = 4 per group, two-tailed t test, ****p < 0.0001. F) Immunoblots of whole brain lysates obtained from 6-month-old Fyn knockout (FynKO) mice and WT controls. G) Quantification of immunoblots in (F). Mean±s.e.m, n = 4 per group, two-tailed t test, *p < 0.05.