Table 2.
Putatively identified metabolites for discrimination between Tg-ctrl and WT-ctrl and between Tg-TRF and Tg-ctrl
| Putative ID | ESI | Formula | m/z | rt | Fold change | Identifier | |
| Tg-ctrl versus WT-ctrl | Tg-TRF versus Tg-ctrl | ||||||
| Hippocampus | |||||||
| N-Aspartic acid | + | C4 H7 N O4 | 134.0444 | 1.020 | ↓ | ↑ | 462 (mzCloud) |
| N-Acetylaspartic acid | – | C6 H9 N O5 | 174.03961 | 0.964 | ns | ↑ | 1376 (mzCloud) |
| L-Tyrosine | – | C9 H11 N O3 | 180.06551 | 1.440 | ↓ | ↑ | 2255 (mzCloud) |
| L-Glutamic acid | – | C5 H9 N O4 | 146.0448 | 0.510 | ↓ | ↑ | 470 (mzCloud) |
| DL-Glutamine | + | C5 H10 N2 O3 | 147.0759 | 0.497 | ↓ | ns | 2967 (mzCloud) |
| L-Phenylalanine | + | C9 H11 N O2 | 166.08585 | 2.255 | ↓ | ns | 8 (mzCloud) |
| L-Histidine | – | C6 H9 N3 O2 | 154.06114 | 0.466 | ns | ↑ | 473 (mzCloud) |
| Pyroglutamate | + | C5 H7 N O3 | 130.0495 | 0.498 | ↓ | ns | 1831 (mzCloud) |
| Creatinine | + | C4 H7 N3 O | 114.06602 | 0.516 | ns | ↑ | 375 (mzCloud) |
| AMP | + | C10 H14 N5 O7 P | 348.0689 | 1.068 | ↓ | ↑ | 252 (mzCloud) |
| IMP | – | C10 H13 N4 O8 P | 347.03943 | 1.318 | ns | ↑ | 452 (mzCloud) |
| UMP | – | C9 H13 N2 O9 P | 323.02802 | 0.952 | ns | ↑ | 1405 (mzCloud) |
| Adenosine | + | C10 H13 N5 O4 | 268.10309 | 1.952 | ↑ | ns | 297 (mzCloud) |
| Adenine | + | C5 H5 N5 | 136.0614 | 0.781 | ↓ | ↑ | 296 (mzCloud) |
| Inosine | + | C10 H12 N4 O5 | 269.08713 | 2.025 | ↑ | ns | 1234 (mzCloud) |
| Guanine | + | C5 H5 N5 O | 152.05634 | 1.244 | ns | ↓ | 436 (mzCloud) |
| Uridine | – | C9 H12 N2 O6 | 243.06168 | 1.641 | ns | ↑ | 1408 (mzCloud) |
| Desmosterol | + | C27 H44 O | 385.34525 | 10.026 | ↓ | ns | HMDB02719 |
| Glycerophosphocholine | + | C8 H20 N O6 P | 258.10947 | 0.566 | ↑ | ns | HMDB00049 |
| Glycerylphosphorylethanolamine | + | C5 H14 N O6 P | 216.06259 | 0.517 | ↑ | ns | HMDB00114 |
| Ceramide (d18:0/18:1(11Z)) | + | C36 H71 NO3 | 566.54926 | 9.672 | ns | ↓ | HMDB11762 |
| Galabiosylceramide (d18:1/20:0) | + | C50 H95 N O13 | 918.68713 | 13.334 | ns | ↓ | HMDB04835 |
| PS(22:1(11Z)/0:0) | + | C28 H54 N O9 P | 580.36005 | 6.799 | ↑ | ↓ | LMGP03050023 |
| LysoPE(16:0) | + | C21 H44 N O7 P | 454.29132 | 6.938 | ns | ↓ | LMGP02050036 |
| LysoPE(22:4) | + | C27 H48 N O7 P | 530.32361 | 7.131 | ns | ↓ | HMDB11493 |
| GDP-glucose | – | C16 H25 N5 O16 P2 | 604.06952 | 1.011 | ↓ | ↑ | HMDB03351 |
| Sedoheptulose 7-phosphate | – | C7H15O10P | 289.03262 | 0.562 | ↓ | ↑ | C05382 (KEGG) |
| DL-Malic acid | – | C4 H6 O5 | 133.01306 | 0.711 | ↓ | ↑ | 1796 (mzCloud) |
| Choline | + | C5 H13 N O | 104.10697 | 0.520 | ↑ | ns | 886 (mzCloud) |
| Acetylcholine | + | C7 H15 N O2 | 146.11714 | 0.611 | ↓ | ↑ | 885 (mzCloud) |
| Phosphocholine | + | C5 H14 N O4 P | 184.07281 | 0.541 | ↑ | ns | C00588 (KEGG) |
| S-Adenosylmethionine | + | C15 H22 N6 O5 S | 399.14316 | 0.485 | ns | ↑ | 896 (mzCloud) |
| Spermidine | + | C7 H19 N3 | 146.16478 | 0.365 | ↑ | ns | 9 (mzCloud) |
| γ-Aminobutyric acid | + | C4 H9 N O2 | 104.07059 | 0.475 | ns | ↑ | 796 (mzCloud) |
| Uric acid | – | C5 H4 N4 O3 | 167.02002 | 1.163 | ↓ | ↑ | 753 (mzCloud) |
| Carnosine | – | C9 H14 N4 O3 | 225.09854 | 0.444 | ns | ↑ | C00386 (KEGG) |
| Nicotinamide | + | C6 H6 N2 O | 123.05505 | 1.162 | ns | ↑ | 517 (mzCloud) |
| mPFC | |||||||
| Ceramide (d18:0/14:0) | + | C32 H65 N O3 | 512.50287 | 7.105 | ↑ | ns | 53968 (Metlin) |
| Phosphatidylinositol | + | C45 H87 O12 P | 851.6037 | 8.417 | ↑ | ↓ | Several |
| Phosphocholine | + | C11 H24 N O7 P | 315.14301 | 4.673 | ↑ | ↓ | Several |
| Striatum | |||||||
| γ-Glutamylcysteine | + | C8 H14 N2 O5 S | 251.06882 | 0.949 | ↑ | ns | 427 (mzCloud) |
| γ-L-Glutamyl-L-glutamic acid | + | C10 H16 N2 O7 | 277.1022 | 0.682 | ↓ | ns | 1223 (mzCloud) |
| PS(18:1(9Z)/0:0) | + | C24 H46 N O9 P | 524.29797 | 6.619 | ↓ | ns | 40829 (Metlin) |
| LysoPC(18:1(11Z)) | + | C26 H52 N O7 P | 522.35492 | 6.755 | ↑ | ns | HMDB10385 |
| LysoPE(20:1(11Z)/0:0) | + | C25 H50 N O7 P | 508.3389 | 7.162 | ns | ↑ | HMDB11513 |
| LysoPE(0:0/20:4(5Z,8Z,11Z,14Z)) | – | C25 H44 N O7 P | 500.27817 | 13.450 | ns | ↓ | HMDB11487 |
| Sphingosine | + | C18 H37 N O2 | 300.28873 | 6.084 | ↑ | ↓ | HMDB00252 |
| ADP ribose | – | C15 H23 N5 O14 P2 | 558.0636 | 0.847 | ↑ | ↓ | 1708 (mzCloud) |
| β-D-Fructose 1,6-bisphosphate | – | C6 H14 O12 P2 | 338.98853 | 0.524 | ↑ | ns | C05378 (KEGG) |
| Isocitrate | + | C6 H8 O7 | 193.03407 | 1.126 | ↑ | ↓ | HMDB00193 |
| cis-Aconitate | – | C6 H6 O6 | 173.0083 | 1.577 | ↑ | ↓ | C00417 (KEGG) |
| Adenylosuccinate | + | C14 H18 N5 O11 P | 464.08066 | 2.166 | ↑ | ↓ | HMDB00536 |
| Acetyl-L-carnitine | + | C9 H17 N O4 | 204.12244 | 0.799 | ↓ | ns | 879 (mzCloud) |
| 6β-Prostaglandin I1 | – | C20 H34 O5 | 353.2333 | 6.225 | ↑ | ↓ | 4594 (mzCloud) |
| D-4’-Phosphopantothenate | – | C9 H18 N O8 P | 298.06964 | 0.873 | ns | ↓ | C03492 (KEGG) |
↑, upregulated; ↓, downregulated; AMP, adenosine monophosphate; GDP, guanosine diphosphate; PS, phosphatidylserine; ADP, adenosine diphosphate; PS, phosphatidylserine; LysoPC, lysophosphatidylcholine; UMP, uridine monophosphate; IMP, inosine-5’-monophosphate; LysoPE, lysophosphatidylethanolamine.