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. 2018 Jun 11;11:171. doi: 10.3389/fnmol.2018.00171

Figure 3.

Figure 3

NMDAR blockade impairs miR-134-dependent repression of Pum2. Luciferase reporter gene assays performed in either DIV11 hippocampal neurons (HC) (A) or DIV6 cortical neurons (CTX) (B) treated for 6 h with BDNF in the presence or absence of APV. pGL4-PEST-Pum2-3′UTRlong firefly reporter (WT), pGL4-PEST-Pum2-3′UTRlong with mutated miR-134 binding site firefly reporter (MUT134) or pGL4-PEST empty vector were transfected together with a pGL4-Renilla reporter for internal normalization. Firefly/Renilla activity ratios were calculated for the Pum2 reporters and subsequently normalized to the respective ratio of pGL4-PEST empty. Values represent the mean BDNF repression index (ratio normalized luciferase activity BDNF vs. Mock-treated neurons) ± SD of n = 3 independent biological replicates. Induction indices: two-sample students t-test, *P < 0.05, **P < 0.01. BDNF vs. Mock: one-sample (A) or two-sample (B) students t-test, #P < 0.05.