Fig.2.

Effect of LRSAM1 knockdown on SH-SY5Y cells. A. Double transfection of LRSAM1 siRNA was performed on days one and two of the experimental procedure. Cell counts after every 24 hours from day zero (plating) are depicted. Negative control siRNA, lipofectamine only and untransfected-A cell cultures were performed and used as control for the experiment and B. Microscopy analysis 72 hours after the first LRSAM1 downregulation in double transfected LRSAM1 siRNA and negative control siRNA SH-SY5Y cells. Arrows heads show the formation of early neurites (scale bar: 200 µm).