Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 28;20(3):340–347. doi: 10.22074/cellj.2018.5352

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig.3 — Investigation of apoptotic (caspase-3) and cell cycle (cyclin D1) markers in LRSAM1 knockdown SH-SY5Y cells. Western blot analysis of A. Caspase-3and B. Cyclin D1 levels compared with the controls (negative control siRNA, lipofectamine only and untransfected-A cells). Protein levels were determined 96 hours after the first transfection. ß-ACTIN was used as an internal control. Quantification of caspase-3 and cyclin D1 was performed relative to ß-ACTIN. Untransfected-A cell values (caspase-3 and cyclin D1) were set to 100%. Caspase-3 levels: LRSAM1 siRNA transfected cells (96 ± 5.48%, P<0.018), negative control siRNA transfected cells (100 ± 4.55%, P<0.02), lipofectamine only transfected cells (96 ± 2.58%, P<0.019) and untransfected-A cells (102 ± 5.31%). Cyclin D1 levels: LRSAM1 siRNA transfected cells (44 ± 4.50%, P<0.005), negative control siRNA transfected cells (95 ± 3.99%, P<0.009), lipofectamine only transfected cells (104 ± 3.33%, P<0.03) and untransfected-A cells (99 ± 2.92%).