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. 2018 May 28;20(3):340–347. doi: 10.22074/cellj.2018.5352

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Fig.4 — Evaluation of endogenous and exogenous LRSAM1 levels. A. Western blot analysis of LRSAM1 levels 48 hours after the LRSAM1 transfection in knocked down SH-SY5Y cells. An approximately 30% of endogenous protein was detected. ß-ACTIN was used as an internal control and B. Quantification of ancestral and mutant LRSAM1 was performed relative to ß-ACTIN. Untransfected-B cell values (LRSAM1) were set to 30% (endogenous LRSAM1 background). Ancestral LRSAM1 transfected cells representing ancestral-endogenous + ancestral-exogenous LRSAM1 levels within a single band (89 ± 1.80%, P<0.0087 relative to untransfected-B). LRSAM1 mutant transfected cells representing ancestral-endogenous (31 ± 0.68%, P<0.01) and mutant- exogenous (59 ± 1.68%, P<0.007) LRSAM1 levels in two different size bands. Empty vector pIRES2-EGFP transfected cells representing ancestral- endogenous levels (28 ± 0.71%, P<0.0045). Untransfected-B cells representing ancestral-endogenous LRSAM1 levels (30 ± 0.72%, P<0.0019).