Table 1.
Intervals | Procedure | Counting |
---|---|---|
Day 0 | Platting | √ |
Day 1-40% cells confluency | 1st transfection | √ |
Day 2-24 hours after 1st transfection | 2nd transfection | √ |
Day 3-48 hours after 1st-24 hours after 2nd transfection | Incubation | √ |
Day 4-72 hours after 1st-48 hours after 2nd transfection | Incubation | √ |
Day 5-96 hours after 1st-72 hours after 2nd transfection | Incubation | √ |