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. 2018 May 28;20(3):388–395. doi: 10.22074/cellj.2018.5514

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Fig.1 — Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm).