Fig. 3. New methods for the chemical site-selective modification of engineered Cys on the surface of mAb based on: (a) Julia–Kocieński-like reagents, such as methylsulfonylphenyloxadiazole. (b) Amine-to-thiol coupling using a heterobifunctional reagent, sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate.
