Fig. 6. Methods for the chemical modification of IgGs, antibody fragments and proteins at the N- or C-termini. (a) π-Clamp-mediated antibody conjugation using perfluoroaromatic reagents. (b) Mixed disulfide formation of C-terminal Cys. (c) Thiazolidine modification of N-terminal Cys with aldehyde containing drugs. (d) N-Terminal imidazolidinone formation using 2-pyridinecarboxyaldehyde reagents. TCEP–Tris(2-carboxyethyl)phosphine; DTT–dithiothreitol.
