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PHP1 and PHP2 detect early assemblies of mHTTx1. (A and B) SMC Erenna immunoassays demonstrating capture of misfolded mHTTx1 by PHP1 and PHP2 antibodies over time and detected by the MW8. (C and D) present binding of PHP3 and PHP4 to monomeric soluble MBP-HTTx1 captured by B27, which is lost after initiation of aggregation. S/B ratio was determined by dividing the EP sample values by the EP values from assay buffer.
